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non-linear regression analysis and a four-parameter logistic fit function provided by graphpad prism® 5.04 software  (GraphPad Software Inc)


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    GraphPad Software Inc non-linear regression analysis and a four-parameter logistic fit function provided by graphpad prism® 5.04 software
    Non Linear Regression Analysis And A Four Parameter Logistic Fit Function Provided By Graphpad Prism® 5.04 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CdCl2, ω-CTX but not NiCl2 inhibit IJPs recorded from circular smooth muscle cells. A, IJPs were evoked by a 10 Hz, 1 s stimulus train. These IJPs were blocked by tetrodotoxin (TTX, 0.3 μM) the P2Y1 receptor antagonist, MRS2179 (10 μM) and by the SK channel blocker, apamin (0.1 μM). The stimulus artifacts have been truncated for clarity. B, Concentration inhibition curves for the N-type Ca2+ blocker ω-CTX, and CdCl2 and NiCl2. NiCl2 (up to 50 μM) did not inhibit the IJP (n=4 for each drug). C. Circular muscle IJPs evoked by 5 Hz stimulation are not affected by NiCl2 (50 μM) or NLA (100 μM). D, NiCl2 or NiCl2 plus NLA did not change the amplitude of the single stimuli IJP (n=9). Subsequent addition of apamin (0.1 μM) inhibited the IJP (n=4, *P<0.05 vs. Control).

    Journal: Experimental physiology

    Article Title: R-type Ca 2+ channels couple to inhibitory neurotransmission to the longitudinal muscle in the guinea pig ileum

    doi: 10.1113/EP086027

    Figure Lengend Snippet: CdCl2, ω-CTX but not NiCl2 inhibit IJPs recorded from circular smooth muscle cells. A, IJPs were evoked by a 10 Hz, 1 s stimulus train. These IJPs were blocked by tetrodotoxin (TTX, 0.3 μM) the P2Y1 receptor antagonist, MRS2179 (10 μM) and by the SK channel blocker, apamin (0.1 μM). The stimulus artifacts have been truncated for clarity. B, Concentration inhibition curves for the N-type Ca2+ blocker ω-CTX, and CdCl2 and NiCl2. NiCl2 (up to 50 μM) did not inhibit the IJP (n=4 for each drug). C. Circular muscle IJPs evoked by 5 Hz stimulation are not affected by NiCl2 (50 μM) or NLA (100 μM). D, NiCl2 or NiCl2 plus NLA did not change the amplitude of the single stimuli IJP (n=9). Subsequent addition of apamin (0.1 μM) inhibited the IJP (n=4, *P<0.05 vs. Control).

    Article Snippet: Drug concentration response curves were fit using a 4 parameter (max, min, slope, EC 50 ) non-linear logistic function in GraphPad Prism 6.0 (GraphPad Software, Inc., LaJolla, CA).

    Techniques: Concentration Assay, Inhibition, Control